Cross-linked double-stranded RNA from a defective vesicular stomatitis virus particle
Identifieur interne : 005080 ( Main/Exploration ); précédent : 005079; suivant : 005081Cross-linked double-stranded RNA from a defective vesicular stomatitis virus particle
Auteurs : Jacques Perrault [États-Unis]Source :
- Virology [ 0042-6822 ] ; 1976.
English descriptors
- Teeft :
- Academic press, Cellulose column, Circular molecules, Complementary, Complementary strands, Comprehensive virology, Denatured, Denaturing, Denaturing conditions, Digestion, Dsrna, Dsrna fraction, Dsrna molecules, Electron microscope, Electron microscopy, Encephalomyocarditis virus, Ethanol, Ethanol buffer dsrna fraction, Ethanol precipitation, Extraction procedure, Final concentration, Formamide, Huang, Infectious virions, Initial rnase resistance, Ionic strength, John holland, Limited rnase treatment, Linear molecules, Molecular weight, Molecule, Nucleotide sequences, Perrault, Phosphate buffer, Plenum press, Polymerase activity, Rapid reannealing, Reichmann, Ribonucleic acid synthesis, Rnase, Rnase mixture, Rnase resistance, Room temperature, Salt concentration, Sodium phosphate, Spinco rotor, Ssrna, Stomatitis, Strength conditions, Unusual dsrna, Vesicular, Vesicular stomatitis, Vesicular stomatitis virus, Virion, Virology, Virus particles.
Abstract
Abstract: A class of vesicular stomatitis defective interfering virus particles isolated in this laboratory contains mostly double-stranded RNA of approximately 0.7–0.9 × 106 daltons molecular weight. This RNA “snaps-back” or reanneals independently of concentration after heat denaturation but remains denatured if first nicked with ribonuclease. The nicked denatured products are about half the size of the double-stranded RNA and approximately half of them anneal to infectious virion single-stranded RNA. It is proposed that the complementary strands of this double-stranded RNA are cross-linked to each other, probably covalently. The structure can be viewed best as a hairpin or a closed circle base-paired along most of its length.
Url:
DOI: 10.1016/0042-6822(76)90278-6
Affiliations:
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Le document en format XML
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This RNA “snaps-back” or reanneals independently of concentration after heat denaturation but remains denatured if first nicked with ribonuclease. The nicked denatured products are about half the size of the double-stranded RNA and approximately half of them anneal to infectious virion single-stranded RNA. It is proposed that the complementary strands of this double-stranded RNA are cross-linked to each other, probably covalently. The structure can be viewed best as a hairpin or a closed circle base-paired along most of its length.</div></front></TEI><affiliations><list><country><li>États-Unis</li></country><region><li>Californie</li></region></list><tree><country name="États-Unis"><region name="Californie"><name sortKey="Perrault, Jacques" sort="Perrault, Jacques" uniqKey="Perrault J" first="Jacques" last="Perrault">Jacques Perrault</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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